Core Facilities

Proteomics

Proteomics

Overview

The Proteomics Core Facility provides expertise in biological mass spectrometry for large-scale protein identification and characterization, as well as quantitative proteomics-based workflows. The facility is equipped with high-performance mass spectrometers with electrospray ionization sources coupled to nano-flow liquid chromatographs - ideal for proteomics applications.

Services

Consultation

  • Experimental design
  • Labeling approaches and multiplexing
  • Database searching (MaxQuant)
  • Data filtering and imputation (Perseus)

Routine Protein Identification

  • Immunoprecipitation experiments
  • Purified protein samples

Complex Proteome Characterization

  • with or without relative quantification

Post-Translational Modification Enrichment & Characterization

  • Phosphoproteomics
  • Ubiquitinomics
  • Identification, localization, and quantification

Relative Quantitation Strategies

  • Label Free (LFQ)
  • SILAC
  • SIDL

Primary Sample Processing Approaches

  • s-trap
  • FASP
  • SDS-PAGE & gel digest
  • in-solution

Training is provided for undergraduates, graduate students, post-doctoral scholars (sample processing and database searching).

Major Equipment and Instrumentation

  • Hybrid Quadrupole-Orbitrap Mass Spectrometer (Q Exactive Plus, Thermo) in-line with nanoUHPLC (Ultimate 3000, Thermo)
  • Offline HPLC with Fraction Collection (Ultimate 3000, Thermo)
  • Ingenuity Pathway Analysis Bioinformatics Software - Click Here for Signup Calendar

Fee Schedule

Proteomics Fee Schedule

Other Resources

MaxQuant
Perseus
UniProt

Location and Contact Information

Proteomics Core Facility
Interdisciplinary Research Building
3720 Spectrum Blvd, Suite 309/311
Tampa, FL 33612
E: Dr. Dale Chaput