John J. Kasianowicz

Visiting Research Professor




  • Science management courses (2003) MIT Sloan Management School, Boston, MA
  • Ph.D. (1987) Physiology & Biophysics, SUNY at Stony Brook, Stony Brook, NY.
  • M.A. (1981) Physics, SUNY at Stony Brook, Stony Brook, NY
  • B.A. (1979) Physics, Boston University, Boston, MA (with Distinction)

Professional Appointments

  • Visiting Research Professor, University of South Florida, Tampa, FL (present).
  • Senior Fellow, Freiburg Institute for Advanced Studies, Albert Ludwigs Universität Freiburg, Freiburg, Germany (2021 - 2022)
  • Visiting Research Scientist, University of South Florida, Tampa, FL (2020 - 2022).
  • Adjunct Senior Research Scientist, Columbia University, Dept. of Applied Physics & Applied Mathematics, New York, NY (2014 - present)
  • Leader, Nanobiotechnology Project. National Institute of Standards and Technology (NIST), Gaithersburg, MD (2005 - 2022)
  • Leader, Biomolecular Materials Group, National Institute of Standards and Technology (NIST), Chemical Sciences and Technology Laboratory, Biotechnology Division, Gaithersburg, MD (2000 - March 2004)
  • Visiting Associate Professor. Texas A&M University, Department of Medical Biochemistry and Medical Genetics (May 1997 - January 2005)
  • Physical Scientist/Biophysicist. National Institute of Standards and Technology, Chemical Sciences and Technology Laboratory, Biotechnology Division, Biomolecular Materials Group, Gaithersburg, MD (1994 - 2000)
  • National Academy of Sciences/National Research Council Research Associate. National Institute of Standards and Technology, Chemical Sciences and Technology Laboratory, Biotechnology Division, Biosensors Group, Gaithersburg, MD  (1992 - 1994)
  • Office of Naval Research and NIH IRTA Post-Doctoral Fellow. National Institutes of Health, Laboratory of Biochemistry and Metabolism, NIDDK, Bethesda, MD (1987 - 1992)
  • Instructor of Physics. Eastern Nazarene College, Dept. of Physics & Engineering, Wollaston, MA (1982 - 1983)

Honors & Awards

  • Senior Fellow, Marie Skłodowska-Curie Senior Fellowship/Freiburg Institute for Advanced Studies, Albert-Ludwigs-Universität Freiburg, Freiburg Im Breisgau, Germany (2021 - 2022).
  • Guest Editor of a special issue on Ion Channels in Disease” in Chemical Reviews (2012)
  • Fellow of the American Physical Society, Division of Biological Physics (2010) “for his pioneering contributions to the field of biophysics including the detection, identification, characterization and quantification of biological and chemical polymers, and for the development of a new method for protein structure determination
  • The Department of Commerce Silver Medal (2006) “for pioneering the use of nanopores to electronically probe the structure and function of single biomolecules and for the rapid detection of anthrax toxins
  • Boston University Alumni Award (1979)
  • Boston University College Prize for Excellence in Physics (1979)
  • Grossman Foundation Award (1975)


American Physical Society (1981-present, Fellow 2010; past member and chair of the APSIT); American Chemical Society, American Vacuum Society, Biophysical Society, Materials Research Society, Northern Virginia Astronomy Club, Astronomy Club of Asheville, Central Florida Astronomical Society, Tampa Astronomy Club.

Research and Publications

Research Interests

Our lab pioneered research in nanometer-scale pore-based detection, characterization, and quantification of single molecules.  This work led to two different methods for sequencing DNA, the ability to discriminate between molecules based on size, a technique for single molecule thermodynamics and kinetics, and identifying proteins.

We are currently interested further developing nanopore-based protein identification, elucidating the mechanisms of bacterial virulence, developing novel methods for membrane protein structure determination, and storing and retrieving information in molecules.

Publications in Peer-Reviewed Journals

(14,795 citations; * indicates a selected paper)

Bakshloo, M.A., S. Yahiaoui, F. Piguet, M. Pastoriza-Gallego, R. Daniel, J. Mathé, J.J. Kasianowicz, and A. Oukhaled.  2022.  Polypeptide analysis for nanopore-based protein identification.  Nano Research, 15, 9831-9842 doi:10.1007/s12274-022-4610-1.

* Bakshloo, M.A., J.J. Kasianowicz, M. Pastoriza-Gallego, J. Mathé, R. Daniel, F. Piguet, and A. Oukhaled.  2022.  Nanopore-based protein identification.  J. Am. Chem. Soc. 144, 2716–2725.

Poster, D.L., M.T. Postek, Y.S. Obeng, J.J. Kasianowicz, T.E. Cowan, N.R. Horn, C.C. Miller, and R.A. Martinello.  2022. Models for an ultraviolet-C research and development consortium.  J Res Natl Inst Stan 126, 126055.

* Michelman-Ribeiro, A., K.A. Rubinson, V. Silin, and J.J. Kasianowicz.  2021.  Solution structures of Bacillus anthracis Protective Antigen proteins using Small Angle Neutron Scattering and Protective Antigen 63 ion channel formation kinetics.  Toxins 13, 88.

Poster, D.L., C. Cameron Miller, R.A. Martinello, N.R. Horn, M.T. Postek, T.E. Cowan, Y.S. Obeng, and J.J. Kasianowicz.  2021.  Ultraviolet radiation technologies and healthcare-associated infections: standards and metrology needs. J. NIST Res. 126, 126014.

Wang, H., J.J. Kasianowicz, J.W.F. Robertson, D.L. Poster, and J.H. Ettedgui.  2019.  A comparison of ion channel current blockades caused by individual poly(ethylene glycol) molecules and polyoxometalate nanoclusters.  European Physical Journal E.  42, 83.

Ettedgui, J., J. Forstater, J.W. Robertson, and J.J. Kasianowicz.  2019.  High resolution physical characterization of single metallic nanoparticles.  Journal of Video Experiments.  148, e58257 DOI 10.3791/58257.

Wang, H., J.H. Ettedgui,  J. Forstater, J.W.F. Robertson, J.E. Reiner, H. Zhang, S. Chen, and J.J. Kasianowicz.  2018.  Determining the physical properties of single molecules with nanometer-scale pores.  ACS Sensors 3, 251-263.

Fuller, C.W., S. Kumar, M. Porel, M. Chien, A. Bibillo, P.B. Stranges, M. Dorwart, C. Tao, Z. Li, W. Guo, S. Shi, D. Korenblum, A. Trans, A. Aguirre, E. Liu, T. Harada, J. Pollard, A. Bhat, C. Cech, A Yang, C. Spilman, M. Palla, J.S. Hovis, R. Chen, I. Morozova, S. Kalachikov, J.J. Russo, J. Kasianowicz, R. Davis, S. Roever, G.M. Church, and J. Ju.  2016. Real-time, single molecule electronic DNA sequencing by synthesis using polymer tagged nucleotides on a nanopore array.  Proc. Natl. Acad. Sci. (USA) 113, 5233-5238.

Kasianowicz, J.J. and S.M. Bezrukov.  2016.  On “Three Decades of Nanopore Sequencing”.  Nature Biotechnology 34, 481-482.

* Ettedgui, J.H., J.J. Kasianowicz, and A.K. Balijepalli.  2016.  Single molecule discrimination of heteropolytungstates and their isomers in solution with a nanometer-scale pore.  J. Am. Chem. Soc. 138, 7228−7231.

Silin, V., J.J. Kasianowicz, A. Michelman-Ribero, R.G. Panchal, S. Bavari, and J.W.F. Robertson.  2016.  Biochip for the detection of Bacillus anthracis Lethal Factor and therapeutic agents against anthrax toxins.  Membranes 6, 36.

Forstater, J.F., K. Briggs, J.W.F. Robertson, J.H. Ettedgui, C. Vaz, J.J. Kasianowicz, V. Tabard-Cossa, and A. Balijepalli.  2016.  MOSAIC:  A modular single molecular analysis interface for decoding multi-state nanopore data.  Analyt. Chem. 88, 11900-11907.

Zhu, H., L. Haitao, J. Robertson, A. Balijepalli, S. Krylyuk, A. Davydov, J. Kasianowicz, J. Suehle, and L. Qiliang.  2017.  Novel nanofluidic chemical cells based on self-assembled solid-state SiO2 nanotubes.  Nanotechnology 28, 435601.

Kasianowicz, J.J., A.K. Balijepalli, J.W.F. Robertson, J. Ettedgui, J.H. Forstater, H. Zhang, H. Wang, and J.E. Reiner.  2016.  Modern analytical applications for pore-forming proteins.  Biochimica et Biophysica Acta, Biomembranes 58, 593-606.

Pederson, E., J. Barbalas, B. Drown, M. Culbertson, L. Burden, J.J. Kasianowicz, and D. Burden.  2015.  Proximal capture dynamics for a single biological nanopore sensor.  J. Phys. Chem. Part B: Biophysical Chemistry, Biomaterials, Liquids, and Soft Matter, B: Biophysical Chemistry and Biomolecules.  119, 10448-10455.

Balijepalli, A.K., J.H. Benjamini, A.T. Cornio, J.W. F. Robertson, K.P. Cheung, J.J. Kasianowicz, and C.I. Vaz.  2014.  Quantifying short-lived events in multistate ionic channel current recordings. ACS Nano 8, 1547-1553.

* Reiner, J.E., J.W.F. Robertson, D.L. Burden, L.K. Burden, A. Balijepalli, and J.J. Kasianowicz.  2013.  Temperature sculpting in yoctoliter volumes.  J. Am. Chem. Soc. 135, 3087-3094.

Balijepalli, A., J.W.F. Robertson, J.E. Reiner, J.J. Kasianowicz, and R.W. Pastor.  2013.  Theory of polymer-nanopore interactions refined using molecular dynamics simulations.  J. Am. Chem. Soc. 135, 7064−7072.

* Nablo, B.J., K.M. Halverson, R.G. Panchal, S. Bavari, W. Ribot, A. Friedlander, D. Chabot, T. Nguyen, R. Gussio, J.E. Reiner, J.W.F. Robertson, A. Balijepalli, and J.J. Kasianowicz.   2013.  pH-dependent binding of Bacillus anthracis Lethal Toxin complexes causes artificial lipid membrane destabilization.  J. Chem. Phys. 139, 065101-(1-12).

* Kumar, S., C. Tao, M. Chien, B. Hellner, A. Balijepalli, J.W.F. Robertson, Z. Li, J.J. Russo, J.E. Reiner, J.J. Kasianowicz, and J. Ju.  2012.  Single molecule DNA sequencing by synthesis using PEG-labeled nucleotides and nanopore detection.  Nature Scientific Reports 2, 684 (6 pp.)

* Reiner, J.E., J.W.F. Robertson, A. Balijepalli, D.L. Burden, B.S. Drown, and J.J. Kasianowicz.  2012.  Diffusion limitations of an exonuclease/nanopore-based DNA sequencing engine.  J. Chem. Phys. 137, 214903.

Kasianowicz, J.J.  2012. Bio-inspired nanopore-based sensors: Comment on “Nanopores: A journey towards DNA sequencing” by M. Wanunu.  Physics of Life Reviews 9, 170-171

Robertson, J.W.F., J.J. Kasianowicz, and S. Bannerjee.  2012.  Structure and function of ion channels implicated in disease.  Chemical Reviews 112, 6227-6249.

Reiner, J.E., A.K. Balijepalli, J.W.F. Robertson, J. Suehle, J. Campbell, and J.J. Kasianowicz.  2012.  Disease detection and management via single nanopore-based sensors.  Chemical Reviews 112, 6431-6451.

Prabhu, A.S., K.J Freedman, J.W.F. Robertson, Z. Nikolov, J.J. Kasianowicz, and M. Kim.  2011.  SEM induced shrinking of solid state nanopores for single molecule detection.  Nanotechnology 22, 425302.

Henrickson, S.E., E.A. DiMarzio, Q. Wang, V.M. Stanford, and J.J. Kasianowicz.  2010.  Probing single nanometer-scale pores with polymeric molecular rulers.  J. Chem. Phys. 132, 135101-1-8.

* Reiner, J.E., J.J. Kasianowicz, B.J. Nablo, and J.W.F. Robertson.  2010.  Theory for polymer analysis using nanopore-based single-molecule mass spectrometry.  Proc. Natl. Acad. Sci. (USA) 107, 12080-12085.

Robertson, J.W.F., J.J. Kasianowicz, and J.E. Reiner.  2010.  Ion channel geometry can be resolved to sub-angstrom resolution through single molecule mass spectrometry.  J. Phys. Cond. Matter 22, 454108

* McGillivray, D.J., G. Valincius, I. Ignatiev, F. Heinrich, J.W.F. Robertson, D.J. Vanderah, M. Lösche, and J.J. Kasianowicz.   2009.  Structure of functional Staphylococcus aureus α-hemolysin channels in tethered bilayer lipid membranes.  Biophysical J. 96, 1547-1553.

Scruggs, N.R., J.W.F. Robertson, J.J. Kasianowicz, and K.B. Migler.  2009.   Rectification of the ionic current through carbon nanotubes by electrostatic assembly of polyelectrolytes. Nano Letters 9, 3853-3859.

Hromada, Jr., L.P., B.J. Nablo, J.J. Kasianowicz, M.A. Gaitan, and D.L. DeVoe.  2008.  Polymer bilayer lipid membrane chips with integrated Ag/AgCl electrodes for ion channel measurements. Lab on a Chip 8, 602-608.

Nablo, B.J., K.M. Halverson, J.W.F. Robertson, T.L. Nguyen, S. Bavari, R.G. Panchal, R. Gussio, O.V. Krasilnikov, and J.J. Kasianowicz.  2008.  Probing the Bacillus anthracis PA63 channel with nonelectrolyte polymers.  Biophysical J. 95, 1157-1164.

* Kasianowicz, J.J., J.W.F. Robertson, E.R. Chan, J.E. Reiner, and V.M. Stanford.  2008.  Nanoscopic porous sensors.  Annual Reviews of Analytical Chemistry 1, 737-766.

Culbertson, M.J., J.T.B. Williams, W. Cheng, D.A. Stults, E.R Wiebracht, J.J. Kasianowicz, and D.L. Burden.  2007.  Numerical fluorescence correlation spectroscopy for molecular dynamics analyses under non-standard conditions. Analytical Chemistry 79, 4031-4039.

  • Robertson, J.W.F., C.G. Rodrigues, V.M. Stanford, K. Rubinson, O.V. Krasilnikov, and J.J. Kasianowicz.  2007.  Single molecule mass spectrometry in solution using solitary nanopores.  Proc. Natl. Acad. Sci. (USA) 104, 8207-8211

McGillivray, D.J., G. Valincius, D.J. Vanderah, W. Febo-Ayala, J.T. Woodward, F. Heinrich, J.J. Kasianowicz, and M. Lösche.  2007.  Molecular-scale structural and functional characterization of sparsely tethered bilayer membranes.  Biointerphases 2, 21–33.

Valincius, G., D.J. McGillivray, W. Febo-Ayala, D.J. Vanderah, J.J. Kasianowicz, and M. Lösche.  2006.  Enzyme activity to augment the characterization of tethered bilayer membranes.  J. Phys. Chem. B 110, 10213-10216.

Kasianowicz, J.J., T.L. Nguyen, and V.M. Stanford.  2006.  Enhancing molecular flux through nanopores via attractive interactions.  Proc. Natl. Acad. Sci. (USA) 103, 11431-11432.

Panchal, R.G., K.M. Halverson, W. Ribot, T. Kenny, D. Lane, T.G. Abshire, J.W. Ezzell, B. Powell, J.J. Kasianowicz, and S. Bavari. 2005. Purified anthrax lethal toxin complex exhibits functional and biological activity.  J. Biological Chemistry 280, 10834-10839.

Shenoy, D.K., W. Barger, A. Singh, R.G. Panchal, M. Misakian, V.M. Stanford, and J.J. Kasianowicz.  2005.  Functional reconstitution of ion channels in polymerizable lipid membranes.  Nano Letters 5, 1181-1185.

* Halverson, K.M., R.G. Panchal, T.L. Nguyen, R. Gussio, S.F. Little, M. Misakian, S. Bavari, and J.J. Kasianowicz.  2005.  Asymmetric blockade of anthrax protective antigen ion channel by lethal factor: anthrax therapeutic sensor.  J. Biological Chemistry 280, 34056–34062.

* Merzlyak, P., M-F.P., Capistrano, A. Valeva, J.J. Kasianowicz, and O.V. Krasilnikov.  2005.  Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesis.  Biophysical J. 89, 3059-3070.

Chandler, E.L., A.L. Smith, L.M. Burden, J.J. Kasianowicz, and D.L. Burden.  2004.  Membrane surface dynamics of DNA-threaded nanopores revealed by simultaneous single-molecule optical and ensemble electrical recording.  Langmuir 20, 898-905.

Guo, L., D. Pietkiewicz, E.V. Pavlov, J.J. Kasianowicz, L.M. Dejean, S.J. Korsmeyer, B. Antonsson, and K.W. Kinnally.  2004.  Mitochondrial apoptosis-induced channel MAC and Bax channels are regulated by cytochrome c. American J. of Physiology Cell Physiology 286, C1109-C1117.

Polk, B., M. Bernard, J.J. Kasianowicz, M. Misakian, and M. Gaitan.  2004.  Micro-electroplating silver on sharp edges toward the fabrication of solid-state nanopores. J. Electrochemical Society 151, C559.

Kasianowicz, J.J.  2004.  Nanopores.  Flossing with DNA.  Nature Materials 3, 355-356. Kasianowicz, J.J.  2003.  Nanometer-scale pores: potential applications for DNA characterization and analyte detection.  Disease Markers 18, 185-191.

Misakian, M. and J.J. Kasianowicz.  2003.  Electrostatic control of ionic current in the α-hemolysin ion channel.  J. Membrane Biology 195, 137-146.

DiMarzio, E.A. and J.J. Kasianowicz.  2003.  Phase transitions within the isolated polymer molecule: coupling of the PTM transition to the helix-random coil, the collapse, the adsorption, and the equilibrium polymerization transitions.  J. Chemical Physics 119, 6378-6387.

* Ambjörnsson, T., S.P. Apell, Z. Konkoli, E. DiMarzio, and J.J. Kasianowicz.  2002.  Charged polymer membrane translocation.   J. Chemical Physics 117, 4063-4073.

Misakian, M., J.J. Kasianowicz, B. Robertson, and O. Petersons.  2001.  Resistive properties of the Staphylococcus aureus α-hemolysin ion channel.  Bioelectromagnetics 22, 1-7.

Bezrukov, S.M. and J.J. Kasianowicz.  2001.  Neutral polymers in the nanopores of alamethicin and alpha-hemolysin.  Biologicheskie Membrany 18, 453-457.

* Kasianowicz, J.J., S.E. Henrickson, H.H. Weetall, and B. Robertson.  2001. Simultaneous multianalyte detection with a nanopore.  Analytical Chemistry 73, 2268-2272.

Burden, D.L. and J.J. Kasianowicz.  2000.  Diffusion bias and photophysical dynamics of single molecules in unsupported lipid bilayer membranes probed with confocal microscopy.  J. Physical Chemistry B 26, 6103-6107.

* Henrickson, S.E., M. Misakian, B. Robertson, and J.J. Kasianowicz.  2000.  Driven asymmetric DNA transport in a nanometer-scale pore.  Physical Review Letters 85, 3057-3060.

Glazier, S.A., D.J. Vanderah, A.L. Plant, H. Bayley, G. Valincius, and J.J. Kasianowicz.  2000. Reconstitution of the pore forming toxin α-hemolysin in phospholipid/thiohexa(ethylene oxide) alkane-supported bilayers.  Langmuir 16, 10428-10435.

* Kasianowicz, J.J., D.L. Burden, L. Han, S. Cheley, and H. Bayley.  1999.  Genetically engineered metal ion binding sites on the outside of a channel’s transmembrane β-barrel. Biophysical J. 76, 837-845.

* Akeson, M., D. Branton, J.J. Kasianowicz, E. Brandin, and D.W. Deamer.  1999.  Microsecond time-scale discrimination between polycytidylic acid and polyadenylic acid segments within single RNA molecules.  Biophysical J. 77, 3227-3233.

Braha, O., B. Walker, S. Cheley, J.J. Kasianowicz, M.R. Hobaugh, L. Song, J.E. Gouaux, and H. Bayley.  1997.  Structure-based design of a heteromeric transmembrane pore.  Chemistry & Biology 4, 497-505.

Bezrukov, S.M. and J.J. Kasianowicz.  1997.  Charge state of an ion channel controls neutral polymer entry into its pore. European Biophysics Journal 26, 471-476.

* Kasianowicz, J.J., E. Brandin, D. Branton, and D.W. Deamer.  1996.  Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. (USA) 93, 13770-13773.

* Bezrukov, S.M., I. Vodyanoy, R.A. Brutyan, and J.J. Kasianowicz.  1996.  Dynamics and free energy of polymers partitioning into a nanoscale pore.  Macromolecules 29, 8517-8522.

Korchev, Y.E., C.L. Bashford, G.M. Alder, J.J. Kasianowicz, and C.A. Pasternak.  1995.  Low conductance states of a single channel are not ‘closed’. J. Membrane Biology 147, 233-239.

* Kasianowicz, J.J. and S.M. Bezrukov. 1995. Protonation dynamics of the α-toxin ion channel from spectral analysis of pH dependent current fluctuations.  Biophysical J. 69, 94-105.

Winterhalter, M., S. Marzinka, H. Bürner, R. Benz, and J.J. Kasianowicz. 1995. Interaction of poly(ethylene glycols) with air-water interfaces and lipid monolayers. Investigations on surface pressure and surface potential.  Biophysical J. 69, 1372-1381.

Walker, B., J.J. Kasianowicz, M. Krishnasastry, and H. Bayley.  1994.  A pore-forming protein with a metal actuated switch.  Protein Engineering 7, 655-662.

* Bezrukov, S.M. and J.J. Kasianowicz.  1993.  Current noise reveals protonation kinetics and number of ionizable sites in an open protein ion channel.  Physical Review Letters 70, 2352-2355.

Walker, B.J., M.V. Krishnasastry, L. Zorn, J. Kasianowicz, and H. Bayley. 1992. Purification and characterization of the α−hemolysin of Staphylococcus aureus expressed in Escherichia coli.  J. Biological Chemistry 267, 10902-10909.

Gabev, E., J. Kasianowicz, T. Abbott, and S. McLaughlin. 1989. Binding of neomycin to phosphatidylinositol 4,5 bisphosphate.  Biochim. Biophys. Acta 979, 105-112.

Kasianowicz, J., R. Benz, M. Gutman, and S. McLaughlin.  1987.  Reply to: Diffusion of protons along phospholipid monolayers.  J. Membrane Biol. 99, 228.

* Kasianowicz, J., R. Benz, and S. McLaughlin.  1987.  How do protons cross the membrane-solution interface? Kinetic studies on bilayer membranes exposed to the protonophore S-13 (5-chloro-3-tert-butyl-2'chloro-4'nitrosalicylanilide).  J. Membrane Biol. 95, 73-89.

Kasianowicz, J., R. Benz, and S. McLaughlin.  1984.  The kinetic mechanism by which CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across membranes.  J. Membrane Biol. 82, 179-190.

Book Chapters and Conference Proceedings

Poster, D.L., Z. Tsinas, J.W. Robertson, I. Pazos, D. Catterton, M.T. Postek, J.J. Kasianowicz, and M. Al-Sheikhly.  2018.  Radiation-induced synthesis and modification of soft nanomaterials for medical applications.  Proc. SPIE 10730, Nanoengineering: Fabrication, Properties, Optics, and Devices XV, 107301K

Kasianowicz, J.J. H. Wang, and J. Ettedgui.  2018.  On the detection, characterization, and identification of single molecules with nanopores.  2018 International Symposium on VLSI Technology, Systems, and Application (VLSI-TSA).

Kasianowicz, J.J., J.W.F. Robertson, J.E. Reiner, S.E. Henrickson, C. Rodrigues, and O.V. Krasilnikov.  2012.  Detecting and characterizing individual molecules with single nanopores. In: Nanopore-based technology: Single molecule characterization and DNA sequencing. Ed. Gracheva, M.  Humana Press, Springer, New York, NY.

Robertson, J.W.F., V. Silin, and J.J. Kasianowicz.  2012.  Integrating biological molecules with electrode surfaces for bioanalytical sensing applications.  2011 International Semiconductor Device Research Symposium (ISDRS 2011). DOI: 10.1109/ISDRS.2011.6135209

Kasianowicz, J.J.  Introduction to Ion Channels and Disease.  2012. Chemical Reviews 112, 6215-6217.

Kim, M., J.W.F. Robertson, and J.J. Kasianowicz. 2009. Single molecule analysis using single nanopores.  In: Nanofluidics. Nanoscience and Nanotechnology. Eds. Edel, J.B. and A.J. deMello. Royal Society of Chemistry Publishing, Cambridge, UK, pp. 113-138.

Reiner, J.E., A. Jahn, L.E. Locascio, M. Gaitan, and J.J. Kasianowicz.  2007.  Liposome characterization with fluorescence cumulant analysis. Proc. SPIE Noise and Fluctuations in Biological, Biophysical, and Biomedical Systems, Florence, ITALY, p. 66020I-1-11

Kasianowicz, J.J., S.E. Henrickson, J.C. Lerman, M. Misakian, R.G. Panchal, K.M. Halverson, S. Bavari, R. Gussio, T. Nguyen, D.K. Shenoy, and V.M. Stanford.  2007.  The detection and characterization of ions, DNA and proteins using nanometer-scale pores. Handbook of Biosensors and Biochips. Eds. R.S. Marks, D.C. Cullen, I. Karube, C.R. Lowe, and H.H. Weetall. J. Wiley & Sons.

Hromada, L., J. Kasianowicz, M. Gaitan, D. Millard, and D.L. DeVoe.  2006.  Integrated microfluidic bilayer lipid membrane (BLM) arrays.  Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference:  10th International Conference on Miniaturized Systems for Chemistry and Life Sciences.  pp. 531-533.

Stanford, V.M and J.J. Kasianowicz.  2004.  Transport of DNA through a single nanometer-scale pore: evolution of signal structure. IEEE Workshop on Genomic Signal Processing and Statistics. 26 May, 2004. Baltimore, MD.

DiMarzio, E.A. and J.J. Kasianowicz.  2003.  Phase transitions of a polymer threading a membrane: character of the transition when the molecule can undergo a helix-random coil or an equilibrium polymerization transition. Unsolved Problems of Noise and Fluctuations in Physics, Biology, and High Technology (UPoN'2002). Ed. S.M. Bezrukov. American Institute of Physics Conference Proceedings 665, pp. 344-354.

Stanford, V. and J.J. Kasianowicz.  2003.  On quantifying signatures from single-stranded DNA driven through nanometer-scale α-hemolysin ion channels using Hidden Markov Models. IEEE Workshop on Genomic Signal Processing and Statistics. 11-13 October, 2002. Raleigh, NC

Deamer, D.W., M.A. Akeson, and J.J. Kasianowicz.  2002.  Mechanism of ionic current blockades during polymer transport through pores with nanometer dimensions. NATO Advanced Research Workshop.  Structure and Dynamics of Confined Polymers. Kluwer Press. Eds. J.J. Kasianowicz, M. Kellermayer and D.W. Deamer. pp. 165-175.

* Kasianowicz, J.J., S.E. Henrickson, M. Misakian, H.H. Weetall, B. Robertson, and V. Stanford.  2002.  Physics of DNA threading through a nanometer pore and applications to simultaneous multianalyte sensing. NATO Advanced Research Workshop.  Structure and Dynamics of Confined Polymers. Kluwer Press. Eds. J.J. Kasianowicz, M. Kellermayer and D.W. Deamer. pp. 141-163.

* Bezrukov, S.M. and J.J. Kasianowicz.  2002.  Dynamic partitioning of neutral polymers into a single ion channel. NATO Advanced Research Workshop.  Structure and Dynamics of Confined Polymers. Kluwer Press. Eds. J.J. Kasianowicz, M. Kellermayer and D.W. Deamer. pp. 117-130.

Burden, D.L. and J.J. Kasianowicz.  2000.  Interrogating unsupported lipid bilayers with scanning confocal single-molecule fluorescence microscopy.  SPIE 3922, 27-36.

Kasianowicz, J., B. Walker, M. Krishnasastry, and H. Bayley.  1994.  Genetically engineered pores as metal ion biosensors.  Materials Research Symposium 330, 217-223.

Bezrukov, S.M. and J.J.  Kasianowicz.  1993.  Noise analysis of ionization kinetics in a protein ion channel.  In: Proceedings of the 12th Int'l. Conference on Noise in Physical Systems and 1/f Noise, edited by P.M. Handel and A.L. Chung, American Institute of Physics Press, NY, pp. 677-680.

Bayley, H., M. Krishnasastry, B. Walker, and J. Kasianowicz.  1993.  Assembly of α-hemolysin: a proteinaceous pore with potential applications in materials synthesis.  Materials Research Symposium 292, 243-252.

Bayley, H., M. Krishnasastry, B. Walker, and J.J. Kasianowicz.  1992.  α−hemolysin, a self-assembling protein pore with potential applications in the synthesis of new materials.  MRS Symp. 255, 205-210.

Krishnasastry, M., B. Walker, L. Zorn, J. Kasianowicz, and H. Bayley.  1992.  Genetically engineered protein pores as components of synthetic microstructures. In: Synthetic Microstructures in Biological Research, edited by J. Schnur and M. Peckerar, NY, Plenum Press, pp. 41-51.


Characterization of individual polymer molecules based on monomer-interface interactions. Church, G., D.W. Deamer, D. Branton, R. Baldarelli, and J. Kasianowicz. USP 5,795,782.  19 August 1998.

Characterization of individual polymer molecules based on monomer-interface interactions. Baldarelli, R., D. Branton, G. Church, D.W. Deamer, M. Akeson, and J. Kasianowicz. USP 6,015,714.  18 January 2000.

Designed protein pores as components for biosensors.  Bayley, H., O. Braha, J. Kasianowicz, and E. Gouaux. USP 6,824,659.  20 November 2004.

Method of stabilization of functional nanoscale pores for device applications.  Shenoy, D.K., A Singh, W.R. Barger, and J.J. Kasianowicz. USP 8,294,007.  23 October 2012.

Systems and methods for controlling temperature of small volumes.  Kasianowicz, J.J., J.E. Reiner, A.K. Balijepalli, J.W. Robertson. D.L. Burden, and L. Burden.  USP 9921174.  20 March, 2018

Single molecule filter and single molecule electrograph, and process for making and using same.  Suehle, J.S., J.J. Kasianowicz, J.W.F. Robertson, A. Balijepalli, and J. Benjamini. US Patent 10,067,118. 4 September, 2018.

Method and preparation of nanopore and uses thereof. Ju, J. S. Kumar, C. Tao, M. Chien, J.J. Russo, J.J. Kasianowicz, and J.W.F. Robertson.  US Patent US 10,246,479 B2 19 April 2019.

Molecular scrivener for reading and writing information data to a macromolecule.  Cheung, K.A., J.W.F. Robertson, and J.J. Kasianowicz.  US Patent 23 November, 2022.


Electrophysiology, monolayer surface pressure and potential measurements, surface plasmon resonance, and physical theory.


  • Molecular Devices Axon Instruments Axopatch 200B patch clamp amplifiers (3)
  • HEKA EPC10-plus double patch clamp amplifier
  • Dagan 3900A Integrating patch clamp amplifier
  • Electronic BioSciences/Quartz nanocapillary patch clamp amplifier
  • Nanion Orbit 16 custom patch clamp amplifier
  • Sutter Instruments P-2000 Laser Pipette Puller
  • KSV Langmuir monolayer apparatus
  • Rank Brothers Mark II Micro electrophoresis apparatus
  • Tektronix 410 Oscilloscopes (2)
  • Tektronix 430 Oscilloscope
  • Carl Zeiss inverted microscopes (2)