Core Facilities

Chemical Purification Analysis and Screening core facility (CPAS)

Laurent Calcul

Laurent Calcul, Ph.D. (Director)

calcul@usf.edu  (preferred method)
Phone NES 406: (813) 974-6031
Phone IDRB 318: (813) 974-6031
Fax: (813) 974-3203

Need a Reservation? Please Check the Instrument availablility below:

OVERVIEW

CPAS offers its expertise in chromatographic separation and purification (MPLC, HPLC, UHPLC), mass spectrometry detection, identification and quantification (LC-MS SQ, LC-MS QqQ, LC-MS ToF, LC-MS QToF, GC-MSD and GC-MS QToF and MALDI TOF). The services also include Circular Dichroism, general spectroscopic characterization (UV, FTIR), lyophilization (benchtop and general purpose freeze-dryer), synthesis (microwave reactor), ADME screening (Parallel Artificial Membrane Permeability [PAMPA] and drug solubility evaluation), and high-throughput screening development capability (liquid handling robot and multimode plate reader). Instruments at CPAS are available 24/7 upon reservation in self-service for users who completed the training.

USF CPAS EQUIPMENT AND SERVICES (NES 406 and IDRB 318)

CPAS, NES 406

Agilent LC-MS QToF 6540

Agilent LC-MS QToF 6540

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Mass Range/Resolution: - Range 40k m/z; Accuracy 20 – 10,000)
  • Spectrum Dynamic range: - 5 order of magnitude
  • Scan Speed: - 50 Spectra/sec
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolomic identification
  • Software: - Mass Hunter Workstation, Mass Profiler Professional (MPP)

 

Agilent LC-MS QqQ 6460

Agilent LC-MS QqQ 6460

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Sensitivity: - High sensitivity (Femtomole to Attomole range)
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolite identification
  • Software: - Mass Hunter workstation
  • Studies: - Target ion capability (Quantitative analysis)

 

Agilent LC-MS SQ 6120

Agilent LC-MS SQ 6120

  • Ionization: - Electrospray (ESI ± ve); Atmospheric pressure chemical ionization (APCI ± ve)
  • Mass range: - 10 – 2000 m/z
  • Inlet system: - Agilent 1100 HPLC with Diode Array Detector (DAD)
  • Analytes: - Organic, peptides, Natural products and non-polar compounds

 

Agilent GC-MS SQ 5977B

Agilent GC-MSD 5977B

  • Ionization: - Electron impact (EI); Chemical ionization (NCI/ PCI)
  • Mass range: - 1.6 – 1050 m/z
  • NIST library 2017
  • Inlet system: - Agilent 7890B GC
  • Analytes: - low molecular weight range, semi-volatile, low-bowling point, non-polar molecules
  • Gerstel MultiPurpose Sampler (MPS):
    • 10uL syringe, Head Space (HS)/ 2.5mL syringe and HS Solid Phase Micro-Extraction (SPME) fiber
    • Dual head set up for simultaneous sample preparation/ injection
    • Thermal Desorption (TDU) and thermal extraction for analysis of gaseous, liquid and solid samples
    • Automated Liner EXchance (ALEX) technology
    • Cooled Injection System (CIS) allowing cryo-focusing trap with LN2

 

Dionex ultimate 3000 UHPLC DAD

Dionex ultimate 3000 UHPLC DAD

  • Detection: - Ultimate 3000 Diode array detector (DAD)
  • UV range: - 190- 800 nm
  • Inlet system: - Thermo Scientific Dionex Ultimate 3000 UHPLC+ focused with automated fraction collector (AFC)
  • Analytes: - wide range of chemical analytes, from small molecules to biomolecules

 

AVIV Model 215 Circular Dichroism Spectrometer

AVIV Model 215 Circular Dichroism Spectrometer

  • Records wavelength, time, temperature, pH and Concentration
  • Wavelength range 160 nm to 850 nm
  • Light Source: - Air cooled, 150 watt suprasil Xenon lamp controlled by a high stability, constant current, DC power supply
  • Cuvette size: - 1 cm and 1 mm path length

CPAS, IDRB 318

Bruker UltraFleXtreme MALDI- TOF/TOF

Bruker UltraFleXtreme MALDI- TOF/TOF

  • Smartbeam II, 2 GHz for MS and 1 GHz for MS/MS
  • FlashDetector™ with 5 GS/s 10 bit digitizer for enhanced dynamic range
  • Resolution > 45,000
  • Analytes: - Protein, peptide, lipids, natural products and polymer
  • Software: - FlexControl, FlexAnalysis, FlexImaging
  • Database: - BioTools, PolyTools