Core Facilities

Chemical Purification Analysis and Screening core facility (CPAS)

Laurent Calcul 
Laurent Calcul, Ph.D. (Director)

calcul@usf.edu
Phone NES 406: (813) 974-6031
Phone IDRB 318: (813) 974-6031
Fax: (813) 974-3203


OVERVIEW


CPAS offers its expertise in chromatographic separation and purification (MPLC, HPLC, UHPLC), mass spectrometry detection, identification and quantification (LC-MS SQ, LC-MS QqQ, LC-MS ToF, LC-MS QToF, GC-MSD and GC-MS QToF and MALDI TOF). The services also include Circular Dichroism, general spectroscopic characterization (UV, FTIR), lyophilization (benchtop and general purpose freeze-dryer), synthesis (microwave reactor), ADME screening (Parallel Artificial Membrane Permeability [PAMPA] and drug solubility evaluation), and high-throughput screening development capability (liquid handling robot and multimode plate reader). The facility provides research lab training and assistance so users can operate any instruments necessary to successfully accomplish their research projects. 

USF CPAS EQUIPMENT AND SERVICES (NES 406 and IDRB 318)

CPAS, NES 406

Agilent LC-MS QToF 6540

Agilent LC-MS QToF 6540

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Mass Range/Resolution: - Range 40k m/z; Accuracy 20 – 10,000)
  • Spectrum Dynamic range: - 5 order of magnitude
  • Scan Speed: - 50 Spectra/sec
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolomic identification
  • Software: - Mass Hunter Workstation, Mass Profiler Professional (MPP)

 

Agilent LC-MS QqQ 6460

Agilent LC-MS QqQ 6460

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Sensitivity: - High sensitivity (Femtomole to Attomole range)
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolite identification
  • Software: - Mass Hunter workstation
  • Studies: - Target ion capability (Quantitative analysis)

 

Agilent LC-MS SQ G1956A

Agilent LC-MS SQ G1956A

  • Ionization: - Electrospray (ESI ± ve); Atmospheric pressure chemical ionization (APCI ± ve)
  • Mass range: - 10 – 1500 m/z
  • Inlet system: - Agilent 1100 HPLC with Diode Array Detector (DAD)
  • Analytes: - Organic, peptides, Natural products and non-polar compounds

 

Agilent GC-MS SQ 5977B

Agilent GC-MS SQ 5977B

  • Ionization: - Electron impact (EI); Chemical ionization (NCI/ PCI)
  • Mass range: - 20 – 1050 m/z
  • Inlet system: - Agilent 7890B GC
  • Analytes: - low molecular weight range, semi-volatile, low-bowling point, non-polar molecules

 

Dionex ultimate 3000 UHPLC DAD

Dionex ultimate 3000 UHPLC DAD

  • Detection: - Ultimate 3000 Diode array detector (DAD)
  • UV range: - 190- 800 nm
  • Inlet system: - Thermo Scientific Dionex Ultimate 3000 UHPLC+ focused with automated fraction collector (AFC)
  • Analytes: - wide range of chemical analytes, from small molecules to biomolecules

 

AVIV Model 215 Circular Dichroism Spectrometer

AVIV Model 215 Circular Dichroism Spectrometer

  • Records wavelength, time, temperature, pH and Concentration
  • Wavelength range 160 nm to 850 nm
  • Light Source: - Air cooled, 150 watt suprasil Xenon lamp controlled by a high stability, constant current, DC power supply
  • Cuvette size: - 1 cm and 1 mm path length

CPAS, IDRB 318

Bruker UltraFleXtreme MALDI- TOF/TOF

Bruker UltraFleXtreme MALDI- TOF/TOF

  • Smartbeam II, 2 GHz for MS and 1 GHz for MS/MS
  • FlashDetector™ with 5 GS/s 10 bit digitizer for enhanced dynamic range
  • Resolution > 45,000
  • Analytes: - Protein, peptide, lipids, natural products and polymer
  • Software: - FlexControl, FlexAnalysis, FlexImaging
  • Database: - BioTools, PolyTools