Chemical Purification Analysis and Screening core facility (CPAS)

OVERVIEW

CPAS offers its expertise in chromatographic separation and purification (MPLC, HPLC, UHPLC), mass spectrometry detection, identification and quantification (LC-MS SQ, LC-MS QqQ, LC-MS ToF, LC-MS QToF, GC-MSD and GC-MS QToF and MALDI TOF). The services also include Circular Dichroism, general spectroscopic characterization (UV, FTIR), lyophilization (benchtop and general purpose freeze-dryer), synthesis (microwave reactor), ADME screening (Parallel Artificial Membrane Permeability [PAMPA] and drug solubility evaluation), and high-throughput screening development capability (liquid handling robot and multimode plate reader). Instruments at CPAS are available 24/7 upon reservation in self-service for users who completed the training.

• Contact & Billing
• Usage Fees
• Information Poster
• Mass Spectrometry Analysis Form
• Acknowledgement Co-Authorship

USF CPAS EQUIPMENT AND SERVICES (NES 406 and IDRB 318)

CPAS, NES 406

Agilent GC-MSD 5977B

• Ionization: - Electron impact (EI); Chemical ionization (NCI/ PCI)
• Mass range: - 1.6 – 1050 m/z
• NIST library 2017
• Inlet system: - Agilent 7890B GC
• Analytes: - low molecular weight range, semi-volatile, low-bowling point, non-polar molecules
• Gerstel MultiPurpose Sampler (MPS):
  • Dual headed for sample preparation
  • 10uL syringe, HS/ 2.5mL syringe and HS SPME fiber
  • Cooled Injection System (CIS) allowing cryo-focusing (LN2)
  • Thermal Desorption (TDU) and Thermal Extraction using Automated Liner Exchance (ALEX) for:
    • Twister/ Stir Bar Sorptive Extraction SBSE
    • Thin Film (TF-SPME)
    • Desorption liner (absorbent for air sampling)

Dionex ultimate 3000 UHPLC DAD

• Detection: - Ultimate 3000 Diode array detector (DAD)
• UV range: - 190- 800 nm
• Inlet system: - Thermo Scientific Dionex Ultimate 3000 UHPLC+ focused with automated fraction collector (AFC)
• Analytes: - wide range of chemical analytes, from small molecules to biomolecules

---

 

AVIV Model 215 Circular Dichroism Spectrometer

• Records wavelength, time, temperature, pH and Concentration
• Wavelength range 160 nm to 850 nm
• Light Source: - Air cooled, 150 watt suprasil Xenon lamp controlled by a high stability, constant current, DC power supply
• Cuvette size: - 1 cm and 1 mm path length

CPAS, IDRB 318

• Agilent 7890A GC-MS QToF 7200
• Agilent 1200 LC-MS TOF 6230
• Agilent 1260 Preparative HPLC-DAD-MS SQ 6120
• Teledyne-Isco MPLC (UV) and Shimadzu HPLC (UV, ELSD, EID)
• Parallel Artificial membrane Permeability Assay (GIT, BBB, Skin PAMPA)
• Solubility evaluation
• High-throughput Screening Capabilities
• Anton Paar Monowave Reactor 300
• Agilent Cary 60 UV-Vis Spectrophotometer and FTIR 630 Spectrometer
• Freeze Drying and Concentration
• Innovatice Technologies Pure Solv Micro solvent purification system for anhydrous solvent preparation

Bruker UltraFleXtreme MALDI- TOF/TOF

• Smartbeam II, 2 GHz for MS and 1 GHz for MS/MS
• FlashDetector™ with 5 GS/s 10 bit digitizer for enhanced dynamic range
• Resolution > 45,000
• Analytes: - Protein, peptide, lipids, natural products and polymer
• Software: - FlexControl, FlexAnalysis, FlexImaging
• Supporting Software: Biotools, Polytools, SCiLS Lab