Chemical Purification Analysis and Screening core facility (CPAS)

OVERVIEW

CPAS offers its expertise in chromatographic separation and purification (MPLC, HPLC, UHPLC), mass spectrometry detection, identification and quantification (LC-MS SQ, LC-MS QqQ, LC-MS TOF, LC-MS QToF, TDU-GC-MSD, GC-MSD-uECD and GC-MS QTOF [HS-SPME] and MALDI TOF). The services also include Circular Dichroism (CD, VCD), Optical Rotatory Dispersion (ORD), general spectroscopic characterization (UV, FTIR), lyophilization (benchtop and general purpose freeze-dryer), extraction (Accelerated Solvent Extactor [ASE], microwave reactor), synthesis (microwave reactor, hydrogenation apparatus), ADME screening (Parallel Artificial Membrane Permeability [PAMPA - GIT, BBB and skin] and drug solubility evaluation), binding affinity measurement (Microscale Thermophoresis [MST]), and high-throughput screening development capability (liquid handling robot and multimode plate reader). CPAS provides trainings and instruments are available 24/7 in self-service mode and upon reservation for trained users.

• Contact & Billing
• Usage Fees
• Information Poster
• Mass Spectrometry Analysis Form
• Acknowledgement Co-Authorship

USF CPAS EQUIPMENT AND SERVICES (NES 406 and IDRB 318)

CPAS, NES 406

Agilent GC-MSD 5977B

• Ionization: - Electron impact (EI); Chemical ionization (NCI/ PCI)
• Mass range: - 1.6 – 1050 m/z
• NIST library 2017
• Inlet system: - Agilent 7890B GC
• Analytes: - low molecular weight range, semi-volatile, low-bowling point, non-polar molecules
• Gerstel MultiPurpose Sampler (MPS):
  • Dual headed for sample preparation
  • 10uL syringe, HS/ 2.5mL syringe and HS SPME fiber
  • Cooled Injection System (CIS) allowing cryo-focusing (LN2)
  • Thermal Desorption (TDU) and Thermal Extraction using Automated Liner Exchance (ALEX) for:
    • Twister/ Stir Bar Sorptive Extraction SBSE
    • Thin Film (TF-SPME)
    • Desorption liner (absorbent for air sampling)

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Dionex ultimate 3000 UHPLC DAD

• Detection: - Ultimate 3000 Diode array detector (DAD)
• UV range: - 190- 800 nm
• Inlet system: - Thermo Scientific Dionex Ultimate 3000 UHPLC+ focused with automated fraction collector (AFC)
• Analytes: - wide range of chemical analytes, from small molecules to biomolecules

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Dionex Accelerated Solvent Extractor (ASE 350)

• Fast extraction from solid and semisolid matrices
• Reduce solvent consumption
• Pressure up to 1500 PSI
• Temperature up to 200oC
• Nitrogen purge
• Up to 24 samples
• Sample cell volume available (22, 100 mL)
• Environmental, natural products chemistry samples 

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JASCO Model J-1500 Circular Dichroism Optical Rotatory Dispersion (CD/ ORD)  spectrometer

• Electronic CD (ECD)/ORD using UV-Vis spectroscopy 
• Simultaneous Multi-Probe: CD, LD, HT, DC, absorbance 
• Walength range 163 to 950 nm
• Light Source: Xenon lamp
• Cuvette: 10 and 1 mm pathlength
• Peltier thermostatted 6-position automatic cell changer
• Microsampling (2 mL, 10 mL samples)
• Capillaries (5 mL samples) for cell in thermostatted Peltier
• Diffuse-reflectance CD (DRCD) for solid state sample measurements
• Determine the absolute configuration of chiral molecules, secondary structure, folding and binding properties of proteins

Biotools ChiralIR-2X™ Vibrational Circular Dichroism (VCD) spectrometer

• Simultaneous IR and VCD signal measurements
• Internal Dual PEM (Photoelastic Modulators) 
• Biocell (solution)
• SyncRoCell (rotating cell ideal for solid state measurements)
• TempCon-2X (Peltier temperature controller for cell)
• Determine the chiral properties of small organic molecules, peptide, proteins 

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CPAS, IDRB 318

• Agilent 7890A GC-MS QToF 7200
• Agilent 1200 LC-MS TOF 6230
• Agilent 1260 Preparative HPLC-DAD-MS SQ 6120
• Teledyne-Isco MPLC (UV) and Shimadzu HPLC (UV, ELSD, RID)
• Pion Inc. Parallel Artificial Membrane Permeability Assay (GIT, BBB, Skin PAMPA)
• Pion Inc. Solubility evaluation
• TECAN Infinite M 1000 Pro microtiter plate reader and Freedom EVO 150 liquid handler
• Anton Paar Monowave Reactor 300
• Agilent Cary 60 UV-Vis Spectrophotometer and FTIR 630 Spectrometer
• Labconco Freezone, SP Scientific General Purpose Freeze Dryer (GPFD), rotary evaporators and other concentrators 
• Innovatice Technologies Pure Solv Micro solvent purification system for anhydrous solvent preparation

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Paar shaker hydrogenation  apparatus model 3921

• Explosion Proof Motor and Switch
• 1L shaker bottle
• Treating chemicals with hydrogen in the presence of a catalyst (60 PSI)
• Catalytic hydrogenation, reduction or condensation for organic synthesis

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Bruker UltraFleXtreme MALDI- TOF/TOF

• Smartbeam II, 2 GHz for MS and 1 GHz for MS/MS
• FlashDetector™ with 5 GS/s 10 bit digitizer for enhanced dynamic range
• Resolution > 45,000
• Analytes: - Protein, peptide, lipids, natural products and polymer
• Software: - FlexControl, FlexAnalysis, FlexImaging
• Supporting Software: Biotools, Polytools, SCiLS Lab

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Nanotemper MicroScale Thermophoresis (MST) Monolith

• Molecular interaction, binding affinity measurements (Kd)
• Fluorescence channels: RED & BLUE
• Dynamic range 1nM to uM
• Up to 24 samples/ run
• Small molecules, proteins, DNA, …
• JOVE MST link.